Fig. 5.

Treating slice cultures with BAPTA-AM and BAPTA analogs results in intraneuronal chelator accumulation.A, Cellular autoradiography of the CA1 region from a 10-μm-thick section of a ¹⁴C-BAPTA-AM-loaded slice culture (40× microscope objective) counterstained with hematoxylin and eosin. The slice was loaded with 100 μm¹⁴C-BAPTA-AM (see Materials and Methods) and fixed with EDC 24 hr after loading. There was a higher density of silver grains over the cell layers (see Results). Scale bar, 40 μm. B, Confocal image of a slice culture similarly loaded with the fluorescent cell-permeant BAPTA analog calcium green-AM (25 μm), fixed after 5 hr with EDC (see Results), and sectioned (10 μm). Distinct localization of the compound was detected in cell layers. Scale bar, 300 μm. C, Higher magnification of an area from the slice in B confirms intraneuronal loading of calcium green. Scale bar, 20 μm. Data in A andB are representative of five experiments per group.