Fig. 6.

Pretreatment with BAPTA-AM temporarily protects cultured slices from OGD. The slices were pretreated with BAPTA-AM (10 or 100 μm, n = 18 and 19 slices, respectively) in a total of 0.5% DMSO or with DMSO alone. Subsequently, they were exposed to 60 min OGD. A, B, Representative series of experiments. A, OGD-induced cell death was significantly lower in both BAPTA-treated groups at 1, 3, and 5 hr after the insult compared with untreated OGD controls (n = 17 slices). B, At 24 hr, however, this protective effect of BAPTA pretreatment was no longer significant (ANOVA followed by Newman–Keuls procedure for multiple comparisons; groups included are in dotted boxes,p values marked on plot). Open symbols, Glucose deprivation without anoxia (DMSO, 10 μm and 100 μm BAPTA-AM groups, n = 9, 6, and 8 slices, respectively). C, Effect of BAPTA-AM on the survival of OGD-challenged neurons over 24 hr. Data were pooled from two series of experiments using 10 μm BAPTA-AM pretreatment (18 slices) and five series of experiments with 100 μm BAPTA-AM (66 slices). Survival ratios were calculated as in Figure 4C. At 3 and 5 hr after OGD, the survival ratios of 10 and 100 μm BAPTA-AM pretreatments were comparable to those resulting from NMDA antagonists. However, unlike with NMDA antagonists, a protective effect of BAPTA was no longer observed at 24 hr. Asterisks indicate significant differences from zero (no treatment effect).