Fig. 8.

Protection by BAPTA-AM is attributable to intracellular Ca²⁺ chelation, not to extracellular Ca²⁺ buffering or to the AM moiety. Slice cultures were challenged with 60 min OGD in the presence of DMSO alone (DMSO; 24 slices), BAPTA-AM (38 slices), BAPTA tetrapotassium salt (K-BAPTA; 5 slices), which is cell-impermeant, and dinitro-BAPTA-AM (DN-BAPTA-AM; 8 slices), which has a negligible affinity for Ca²⁺.Asterisks indicate differences from DMSO groups (ANOVA followed by Newman–Keuls procedure for multiple comparisons).