Table 1.

Effects of OGD, NMDA, and BAPTA-AM on regional neuronal vulnerability

	5 hr (%)	ANOVA result	24 hr (%)	ANOVA result
OGD
CA1	44  ± 3.8		98  ± 5.1
CA3	45  ± 3.9	p  = 0.91	110  ± 7.5	p  = 0.67
DG	43  ± 3.5		82  ± 9.7
OGD + BAPTA-AM
CA1	20  ± 2.5		85  ± 6.1
CA3	14  ± 3.8	p  = 0.41	85  ± 7.6	p  = 0.95
DG	15  ± 4.2		82  ± 6.2
NMDA
CA1	58  ± 3.9		104  ± 4.3
CA3	47  ± 5.6	p  = 0.09	96  ± 9.3
DG	41  ± 4.2		74  ± 4.9*	p  ± 0.02
NMDA + BAPTA-AM
CA1	57  ± 4.9		111  ± 8.9
CA3	50  ± 7.6	p  = 0.44	102  ± 17.9	p  = 0.30
DG	45  ± 6.6		79  ± 15.8

Neurons in the different regions of the hippocampal culture behave similarly when exposed to OGD, NMDA, and BAPTA-AM. Slice cultures were exposed either to 60 min OGD, 60 min OGD in the presence of BAPTA-AM (100 μm), 60 min of NMDA (100 μm), or 60 min of NMDA in the presence of BAPTA-AM. The mean ± SE percentage of neuronal death in each hippocampal region is shown at 5 and 24 hr after the insult (n = 9 slice cultures/group). Differences between means within groups were sought by ANOVA. All exposures and treatments had similar effects on neuronal death in each hippocampal region, thus supporting the rationale for pooling the fluorescence values from the entire slice for analysis. DG, Dentate gyrus.

^*, Significantly different from CA1 in same group (ANOVA with post hoc t test with Bonferroni correction).