Fig. 9.
Regulation of GDNF mRNA in muscle and its receptors in spinal motorneurons. A, Dark-field micrographs of transverse sections through L5 spinal motorneurons reveal that GDNFR-α mRNA (top panels) and RET mRNA (bottom panels) are upregulated in spinal motorneurons 3 d after nerve transection. B, Autoradiogram of an RPA showing that sciatic nerve transection induces GDNF mRNA expression in gastrocnemius muscle. Note, this cRNA probe protects two mRNA fragments of slightly different lengths that are present in both previously characterized GDNF transcripts. Scale bar: A, 134 μm in top panels and 107 μm in bottom panels.