Fig. 1.

Sphere of diffusion of microinjected CREB antisense ODNs into the amygdala (A) and reduction in CREB-positive nuclei by CREB antisense (B–D). A, FITC-labeled CREB antisense was microinjected, and the fluorescence image recorded as detailed in Materials and Methods. CeM, Central amygdaloid nucleus, medial division; CeL, central amygdaloid nucleus, lateral division; BMA, basomedial amygdaloid nucleus, anterior part; BLA, basolateral amygdaloid nucleus, anterior part; Pir, piriform cortex. The microinjection cannula track and the lesion induced by the tip of the cannula are seen within the sphere of FITC diffusion. The schematic anatomical map is adapted from that of Paxinos and Watson (1986).B, The number of CREB-positive nuclei in the amygdala of CREB antisense (AS) versus sense (S) microinjected rats. In both groups CREB antisense was microinjected into the amygdala 14 hr before CTA training; n = 4 in each group. Theasterisk indicates significance for pair comparison in which a Scheffe contrast test was used with an α of 0.05. C, D, Immunohistochemistry of CREB using CREB antibodies in CREB sense (C) and antisense (D) microinjected rats. The selected fields were taken from the region of the CeL, indicated in A; dot, edge of the microinjection cannula-induced lesion.