Table 1.
Strain | Chemotaxis index1-a | Pumping rate1-b | Defecation cycle period1-c | Mating efficiency (%)1-d | ACh levels1-e |
---|---|---|---|---|---|
Wild type (N2) | 0.86 ± 0.04 | 250 ± 30 | 45 ± 3.5 | 100 | 0.35 ± 0.02 |
rab-3(y250) | 0.69 ± 0.05 | 173 ± 13 | 59 ± 9.3 | 9 | 0.44 ± 0.06 |
rab-3(y251) | 0.72 ± 0.06 | 158 ± 15 | 58 ± 8.0 | 24 | 0.35 ± 0.08 |
rab-3(js49) | N.D. | 157 ± 10 | 56 ± 4.3 | 35 | N.D. |
snt-1(md290)* | 0.01 ± 0.02 | 60.3 ± 30 | 141 ± 66 | 0 | 2.07 ± 0.24 |
Means are presented ± SD. N.D., Not determined.
Chemotaxis to isoamyl alcohol in population assays. The index is calculated as the number of animals in the attractant area less the number of animals in the control area divided by the total number of animals in the assay after 60 min (Bargmann et al., 1993).
Pharyngeal pumps per minute on food.
Seconds between defecation cycles.
Efficiency of mating todpy-11(e224) animals as a percentage of wild-type efficiency.
Acetylcholine levels in picomoles per microgram of protein.
Both defecation and pumping rates were erratic in snt-1mutants. Individual animals examined that failed to defecate or pump during the assays were excluded from this analysis. Such animals represent approximately one-third of snt-1 animals.