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Effect of Ca2+-free/EGTA medium on the level of phospho-DARPP-32 in neostriatum. Slices were incubated in control or Ca2+-free/EGTA medium for 20 min.A, Phospho-DARPP-32 was detected using a phosphorylation state-specific mAb (mAb-23). B, The amount of phospho-DARPP-32 was quantitated by densitometry, and the data were normalized to values obtained with control. Data represent mean ± SEM for four experiments. * p < 0.01 compared with control.
