Table 3.

Effect of D2 agonist on the level of phospho-DARPP-32 is not reduced by tetrodotoxin (TTX)

	TTX (−)	TTX (+)
Control	0.686  ± 0.109	1.0003-150
SKF82526	3.282  ± 0.5753-160	5.492  ± 0.7803-165,3-168
SKF82526 + quinpirole	1.716  ± 0.4093-150,3-165	1.968  ± 0.5593-168,3-150

Neostriatal slices were incubated for a total of 20 min in the absence or presence of TTX (1 μm). TTX was added at 0 min, quinpirole (1 μm) at 10 min, and SKF82526 (1 μm) at 15 min of incubation. The amount of phospho-DARPP-32 was quantitated by densitometry, and the data were normalized to values obtained with TTX alone. Data represent means ± SEM for six to seven experiments.

F3-160:  p < 0.01,

F3-150:  p < 0.05 compared with control/TTX (−);

F3-165:  p < 0.05 compared with SKF82526/TTX (−);

F3-168:  p < 0.01 compared with control/TTX (+);

* p < 0.01 compared with SKF82526/TTX (+).