Fig. 10.

Ratio of layers 2/3/layer 4 NMDAR1 immunostaining intensity compared across ages and experimental conditions in cats (±SD). Average immunostaining levels were calculated for each layer from digitized images, and ratios were determined. A, Comparison of developmental changes in the ratios for normally reared or dark-reared animals. Layers 2/3 are consistently darker than layer 4 at most ages except between P34 and 53, when the two layers are equivalent in intensity (A). There is no significant difference in immunostaining ratios between normal animals and age-matched dark-reared animals or dark-reared animals exposed to light.B, Ratios for normally reared and monocularly TTX-treated cases shown in histogram form. Immunostaining intensity ratios do not vary significantly between normal P53 animals and control animals receiving monocular injections of citrate between P40 and P53 (p < 0.15) (B) or from the columns pertaining to the unblocked eye (p< 0.005). However, note that immunostaining ratios obtained from the columns of the TTX-injected eye are significantly greater than those from normal animals (p < 0.00005; two-tailed t test).