Fig. 7.

Developmental changes in laminar patterns of NMDAR1 immunostaining occur over the same time period throughout cat neocortex. A, Primary auditory cortex at P53.B, Primary auditory cortex at P53 in an animal dark-reared from shortly after birth to P53. Layers 4 and 2/3 are more intensely immunoreactive than the lower layers, and dark-rearing does not affect this pattern of staining. C, Primary somatosensory cortex of normal animal aged P104. D, Primary somatosensory cortex in an animal dark-reared to P104. At this age, somatosensory cortex exhibits relatively intense staining for NMDAR1 in layers 2/3 but not in the other layers. These same laminar-specific patterns of immunostaining are seen in visual cortex at the same ages (compare with Figs. 5A, 6E). Scale bar, 300 μm.