Table 1. Primers used in this study.

Locus-specific primers were synthesized with linker sequences to allow for two-stage PCR amplification and incorporation of sample-specific barcodes, as described in the text. Primer 806R is 18-fold degenerate, and variants were synthesized as a pool as well as individually. Access Array primer sequences, synthesized by Fluidigm (PE1-CS1 and PE2-[BC]-CS2), are shown in Fig. 2.

341F primer	Primer sequence	Linker (CS1) sequence	Final sequence name	Final sequence ordered
341F	CCTACGGGAGGCAGCAG	ACACTGACGACATGGTTCTACA	>CS1_515F	ACACTGACGACATGGTTCTACACCTACGGGAGGCAGCAG