Figure 2.

X-ray μCT of interfacial scaffolds showing demineralization front morphology. High densities are shown in white (mineral), and lower densities are shown in yellow and red (collagen). (a) Fully mineralized bone biopsy prior to submersion in demineralization solution. (b) Interfacial scaffold after half submersion for 4.5 hours in demineralization solution. Demineralization front forms conical profile as demineralization progresses. (c) Fully demineralized bone biopsy. (d – h) Single slices from μCT of (b). Hash mark on Demineralization Front arrow in (b) indicates approximate location of each slice.