Figure 6.
Images and Raman-derived line scan showing local compositional gradient within transitional zone between mineralized and demineralized tissue. (a) Von Kossa stain of transitional zone near middle of a bone plug. (b) White light image showing a partially demineralized individual trabeculae. Red box indicates region from which a Raman line scan was collected. (c) Representative line scan of mineral:matrix ratio, derived from peak intensity ratio of 957 cm−1: 1673 cm−1 from Raman spectroscopy.