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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Mech Dev. 2019 Mar 20;156:20–31. doi: 10.1016/j.mod.2019.03.002

Fig. 5.

Fig. 5.

DNagk promotes expression of the Wg target gene sens in Drosophila. (A-I) RNAi-mediated knockdown of the y control or Nagk in the posterior compartment of third instar wing imaginal discs using the hh-Gal4 driver. The region of hh-Gal4 activity is indicated by expression of Engrailed (En) (green in B, C, E, F, H, I). Knockdown of y (A–C) does not affect sens expression (magenta, A and C), whereas knockdown of Nagk using two independent RNAi lines (D–G) reduces sens expression in the posterior compartment of the discs (D, F, G and I). (J–Q) RNAi-mediated knockdown of Nagk with the C96-Gal4 driver (M–O, Q) reduces sens expression in larval imaginal discs (magenta, M and O) and results in formation of aberrant adult wing notches (Q), whereas RNAi-mediated knockdown of the control y affects neither sens expression (J) nor formation of the wing margin (P). 33% (n = 60) of adult males expressing the Nagk RNAi display wing notches compared to 0% (n = 36) of males expressing the control y RNAi. wg expression is not reduced upon knockdown of Nagk (green, N). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)