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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Mech Dev. 2019 Mar 20;156:20–31. doi: 10.1016/j.mod.2019.03.002

Fig. 6.

Fig. 6.

Viability of Wnt-dependent intestinal enteroids is sensitive to changes in the UDP-GlcNAc salvage pathway. Wnt-dependent intestinal enteroids grown in the presence of increasing concentrations of (A) 3-OMe-GlcNAc or (B) GlcNAc demonstrate decreased and increased viability, respectively. (C) The inhibitory effect of 3-OMe-GlcNAc could be reversed by addition of GlcNAc and (D) the GSK3 inhibitor, CHIR99021, which activates the Wnt pathway by stabilizing β-catenin. (A–D) Graphs (Left) with representative images from three independent replicates (Right). Viability Index is relative to no drug control (100%). Scale bars, 200 μm. Statistical analyses were performed using the GraphPad Prism version 5 software tool (La Jolla, CA, USA). Data shown are mean values ± SD (n = 3) determined by one-way analysis of variance (ANOVA) followed by Bonferroni multiple comparison testing. *p < 0.05 and ***p < 0.001.