Fig. 1.

Detection of NAD⁺ in total RNA extract from Arabidopsis seedling. Total RNAs were digested with P1. The digest was separated by HPLC, and the fraction containing NAD⁺ was collected and analyzed by LC-MS. (A) Representative LC-MS chromatograph of the NAD⁺ standard, the NAD⁺ fraction from the P1-digested RNAs, and the control sample (the RNA sample treated with heat-inactivated P1). The experiment was repeated 3 times with similar results. (B) Product ions from NAD⁺ of the P1 digest were identical to those from the NAD⁺ standard.