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. 2019 May 28;116(24):12035–12044. doi: 10.1073/pnas.1821775116

Fig. 3.

Fig. 3.

NLGN1 S839 phosphorylation attenuates PSD-95 binding. (A and B) GST-NLGN1 fusion proteins were incubated with adult rat brain P2 fraction lysates for GST pulldown experiments. PSD-95/GST-NLGN1 fusion proteins were analyzed. Graph indicates mean ± SEM (n = 3 to 4). ***P = 0.0002 and ****P = 0.0001. (C and D) HA-NLGN1 (WT, S839A, S839E, or ∆PDZ) and PSD-95-myc were transfected in HEK293 cells as indicated in the figures. PSD-95-myc in the immunoprecipitates with HA antibody were analyzed by immunoblotting. The ratio of coimmunoprecipitated PSD-95-myc/immunoprecipitated NLGN1 was analyzed. Graph indicates mean ± SEM (n = 3 to 5). **P = 0.001824 for C. **P = 0.003735 and ****P = 0.0001 for D. (E) HA-NLGN1 (WT, S839A, or S839E) and PSD-95-myc were transfected in cultured cortical neurons. Graph indicates mean ± SEM (n = 3). **P = 0.005729. The statistical significance between the mean of WT and the mean of each condition was calculated using one-way ANOVA with Dunnett’s multiple comparison test.