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. 2019 Mar 27;17(7):1446–1457. doi: 10.1111/pbi.13070

Figure 5.

Figure 5

IDD16 negatively regulates stomatal development. (a) Confocal microscopy image of IDD16pro:IDD16‐ GFP showing nuclear localization of IDD16 in adaxial epidermis of cotyledons in 1‐DAG seedlings. Epidermal cell periphery is visualized by propidium iodide (red) staining. White circles indicate meristemoids (Ms). (b and c) Compared to wild‐type (b), IDD16pro:IDD16‐ GFP transgenic line (c) showed reduced stomatal density in abaxial epidermis of the cotyledons from 8‐DAG seedlings. Scale bars: 50 μm. (d) The expression levels of IDD16 in 4‐DAG wild‐type and IDD16‐RNAi seedlings were determined by qRT‐PCR. Values are means ± SE from three biological replicates. (e–g) Compared to wild‐type Col (e), IDD16‐RNAi plants (f, g) showed increased stomatal density in abaxial epidermis of the cotyledons from 8‐DAG seedlings. Scale bars: 50 μm. (h) Stomata density obtained from the samples in (e–g). Three areas in the midleaf region were imaged per leaf. Data are the mean of six individual plants (mean ± SE, n = 6). Asterisks indicate statistical significance based on Student's t test; ** P < 0.01.