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. 2019 May 20;116(24):11786–11795. doi: 10.1073/pnas.1811827116

Fig. 4.

Fig. 4.

Immune marker expression in placental Cdx2-eGFP cells. (A and B) Flow cytometry analysis of e18 placental Cdx2-eGFP cells demonstrated very low expression of MHC class I (1.1% ± 0.51; n = 3) and class II (0.466% ± 0.16; n = 3) molecules compared with the mouse splenocytes (MHC class I: 65.3 ± 4.8, MHC class II: 72.5 ± 2.5) as a positive control. Data are represented as mean ± SEM (n = 3). SSC-A, side scatter area. ***P = 0.0004, ****P < 0.0001. (C–H) Sorted Cdx2-eGFP cells were analyzed for the expression of 84 genes involved in innate and adaptive immunity. Low expression of CXCR3 [cognate ligand of cxcl10 panel (F) and surface H2-T23 (C–E)]. G and H show an increase in stat3/4 messenger RNA with negligible expression of TLRs and ly96 on sorted Cdx2 cells. Data are represented as mean ± SEM (n = 3).