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Evaluation of three STU‐Cas9 systems with multiplexed two sgRNAs in rice transgenic lines. (a) Schematics of the STU‐Cas9 expression constructs. (b) Upper panel: a summary table of mutation frequencies at two target sites by three STU‐Cas9 systems; lower panel: a schematic presentation of the genotyping results for all T0 lines categorized as biallelic mutation, monoallelic mutation and wild type.
