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STU‐Cas9‐tRNA system with multiplexed six sgRNAs in rice transgenic lines. (a) Schematics of the STU‐Cas9‐tRNA expression construct. (b) Upper panel: a summary table of mutation frequencies at three target sites by the STU‐Cas9‐tRNA system; lower panel: a schematic presentation of the genotyping results for all T0 lines categorized as biallelic mutation, monoallelic mutation and wild type.
