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. 2019 Jan 17;17(7):1431–1445. doi: 10.1111/pbi.13068

Figure 9.

Figure 9

A STU‐Cas12a system for plant genome editing. (a) Schematics of the STU‐Cas12a system expressing one crRNA, which is flanked by direct repeats (DRs) for processing by Cas12a. (b) Mutation frequencies at four target sites by the STU‐Cas12a system. The experiments were carried out in rice protoplasts and the frequencies were measured by amplicon‐based deep sequencing. Error bars represent standard deviations of two biological replicates. (c) Mutation frequencies at four target sites by the STU‐Cas12a system. (d) Schematics of the STU‐Cas12a system expressing four crRNAs, which are flanked by DRs for processing by Cas12a. (e) Mutation frequencies at four target sites by the multiplexed STU‐Cas12a system. The experiments were carried out in rice protoplasts and the frequencies were measured by amplicon‐based deep sequencing. Error bars represent standard deviations of two biological replicates. (f) Upper panel: a summary table for mutation frequencies at four target sites; lower panel: a schematic presentation of the genotyping results for all T0 lines categorized as biallelic mutation, monoallelic mutation and wild type.