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. 2019 May 22;116(24):11906–11915. doi: 10.1073/pnas.1818488116

Fig. 1.

Fig. 1.

Visualization of γδ T lymphocytes from PBMC scRNA-seq requires high resolution and clustering depth t-SNE plots of large datasets. (A) Unsupervised hierarchical clustering and correspondingly colored t-SNE plot at low resolution (granularity 0.6) of 12k PBMC partitions eight clusters lacking a γδ T lymphocyte cluster. (B) Expression levels of the specified biomarker genes (purple) on the same t-SNE plot. (C) Unsupervised hierarchical clustering and correspondingly colored t-SNE plot at high resolution (granularity 1.2) of the same dataset partition 17 clusters, of which one (boxed area) comprises cytotoxic CD8 T and γδ T cells. (D) Zoom-in of the boxed region (Top Right) with expression levels (purple) of the specified genes.