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. 2019 May 22;116(24):11906–11915. doi: 10.1073/pnas.1818488116

Fig. 4.

Fig. 4.

Distinctive gene-expression patterns of TCRVδ1 and TCRVδ2 γδ T lymphocytes from CMV+ donor #1. (A) Zoom-in of the same cytotoxic cell area of t-SNE plot as in Fig. 1, shown with purple color-encoded expression level of NKG7 gene across the NK cells, CD8 T lymphocytes, TCRVδ1, and TCRVδ2 γδ T lymphocytes. (B) Same as in A for expression of the specified genes. (C) Representative genes differentially expressed between TCRVδ1 and TCRVδ2 γδ T lymphocytes. (D) Functional enrichment of the specified signatures in each single cell from the integrated PBMC and purified γδ T cells dataset is shown by featuring the single-cell enrichment score (i.e., the proportion of UMI for genes from the signature and expressed by each cell divided by the total UMI of the cell) (SI Appendix, Material and Methods), and contour plots of the region with differentially enriched signature over the t-SNE plot (region of cells having signature score >2× mean score of t-SNE). They comprise a negative control signature (12 randomly selected genes, RGS407_12 from this study), B cell activation [132 genes, gene ontology (GO)], dendritic cell differentiation (33 genes, GO), NK cell activation (55 genes, GO), regulation of fever generation (11 genes, GO), regulation of macrophage chemotaxis (16 genes, GO), positive regulation of T cell cytotoxicity (16 genes, GO), cytosolic DNA sensing: (56 genes, Kyoto Encyclopedia of Genes and Genomes). The corresponding genes are listed in SI Appendix, Table S6.