Figure 4. TFEB/TFE3‐mediated regulation of Rev‐erbα contributes to the oscillation of gene expression.

1. Venn diagram depicting the unique and shared TFE3 and REV‐ERBα binding peaks identified as reported in the Materials and Methods section. Fisher's exact test P‐value < 2.2e‐16.
2. KEGG analysis showing that shared TFE3 and REV‐ERBα peaks are enriched for genes involved in lysosome and metabolic pathway.
3. Average TFE3 and REV‐ERBα signals from −1 kb to +1 kb to the transcriptional start site (TSS).
4. TFE3 cistromes significantly overlap with REV‐ERBα in the promoter region of genes involved in autophagy and lysosome. Tag counts are shown in the corner.
5. E‐boxes/CLEAR (red) sites and RORE (green) location in the promoter of their target genes. Arrows indicate the number from transcriptional start site.
6. Expression analysis of autophagy‐related genes in Hepa1‐6 overexpressing TFEB or TFE3 and depleted for Rev‐erbα (n = 3 per group). mRNA levels were normalized using Rps16 and expressed as relative to cells transfected with scramble siRNA and empty vector. Data are represented as mean ± SEM (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 by Student's t‐test).
7. Western blot analysis of Hepa1‐6 overexpressing TFEB or TFE3 and depleted for Rev‐erbα (n = 3 per group). Cells were treated with bafilomycin for 4 h before collection to analyze the autophagy flux. Data are presented as mean ± SEM. (two‐way ANOVA test followed by the Bonferroni post hoc test: *P ≤ 0.05).

Source data are available online for this figure.