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. 2019 May 6;38(12):e101452. doi: 10.15252/embj.2018101452

Figure EV4. ANO8 markedly increases current activated by STIM1(ΔK) that is PI(4,5)P2‐dependent.

Figure EV4

  • A
    Current density recorded with pipette solution containing 10 mM BAPTA in cells transfected with STIM1(ΔK), Orai1 and with (red) or without (black) ANO8.
  • B, C
    STIM1(ΔK) puncta were recorded with TIRF microscopy of cells expressing STIM1(ΔK), Orai1, and with or without ANO8. Example images are shown in (B) and the average of the indicated number of cells in (C).
  • D
    HEK cells expressing STIM1(ΔK), Orai1, ANO8, FRB, and FKB were used to measure CRAC current without (black) and with treatment with 0.2 μM rapamycin to deplete PI(4,5)P2 (red).