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. 2019 Apr 26;38(12):e102099. doi: 10.15252/embj.2019102099

Figure 1. Engineering of split‐Cre and split‐tTA effector molecules.

Figure 1

  1. Schematic depiction of bronchioalveolar cell types and strategy for selective in vivo manipulation of dual‐marker expressing target cells.
  2. Split‐effector construct design.
  3. Quantification of Cre activity by dual luciferase assays (mean ± SD, n = 3).
  4. Quantification of tTA activity by dual luciferase assays [mean ± SD, n = 3 (N and C) or n = 7 (N/C)].
Data information: Npu, Nostoc punctiforme; Ssp, Synechocystis sp.; CDS, coding sequence; BASCs, bronchioalveolar stem cells; BADJ, bronchioalveolar duct junction; NLS, nuclear localization signal.Source data are available online for this figure.