Bronchioalveolar stem cells are a main source for regeneration of distal lung epithelia in vivo

A, B

Scheme of engineered Spc and Ccsp loci after integration of split‐Cre and split‐tTA effectors (red mark = stop codon) and comparison of endogenous SPC or CCSP expression and YFP or mCherry reporter expression in lung sections. Blue: DAPI. Scale bar: 100 μm.

C, D

β‐galactosidase and H&E staining of lung sections from BASC tracer and BASC viewer animals. Scale bars: 500 μm (overview) and 20 μm (boxed magnification).

E

Localization of β‐gal⁺ cells in adult BASC viewer animals. BADJ‐associated and “alveolar” β‐gal⁺ cells are expressed as percentage of all lineage‐labeled cells (mean ± SD, n = 3).

F

Number of β‐gal⁺ cells in adult BASC tracer and BASC viewer animals, normalized to the number of terminal bronchioles (TBs; mean ± SD, n = 3).

Figure 2. Expression of split‐effectors from Spc and Ccsp loci targets BASCs at BADJs.