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Viable single cells of non‐endothelial and non‐hematopoietic origin (CD31/CD45 neg) derived from lungs of BASC viewer mice were analyzed for Sca‐1 immunoreactivity to establish the gates Sca‐1 neg/low/high. Sca‐1 fractions were subsequently analyzed for mCherry and YFP fluorescence to identify Club cells (mCherry+), AT2 cells (YFP+), and BASCs (2+).
