Figure 6. BASCs contribute substantially to lung regeneration and display multi‐lineage differentiation potential.

1. β‐galactosidase staining of cleared whole lung preparations isolated from control and injured BASC v‐race animals 21 days (bleomycin, naphthalene) or 35 days (influenza) following injury. Scale bar: 5 mm (whole lungs) and 1 mm (magnification).
2. Sequential microscopic imaging of epifluorescence and β‐galactosidase activity in lung sections of control and injured BASC v‐race mice. Clusters of BASC‐derived AT2 (YFP⁺) and Club cells (mCherry⁺) are highlighted. Blue: DAPI. Scale bar: 200 μm.
3. Contribution of BASCs to different cellular compartments. Bronchiolar and alveolar accumulations of lineage‐traced BASC derivatives are expressed as percentage of all clusters containing ≥ 10 β‐gal⁺ cells. Data depicted as mean ± SD, n = 4 (bleomycin, naphthalene) or n = 5 (influenza).

Source data are available online for this figure.