Fig. 3.
Flow cytometry analysis of neoPG (a) cell surface incorporation and (b) cell surface retention. A dose-dependent increase in fluorescence is observed with increasing concentrations of a lipid-terminated polymer (GlcNAc6S-lipid), whereas azide-terminated polymers (GlcNAc-6S-azide) displayed minimal fluorescence. Greater than 50% of fluorescence is retained after 8 h following glycocalyx remodeling. Reprinted with permission from Huang, M.L., Smith, R.A.A., Trieger, G.W., Godula, K. Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells. Journal of the American Chemical Society 136, 10565–10568. Copyright (2014) American Chemical Society