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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Methods Mol Biol. 2016;1367:207–224. doi: 10.1007/978-1-4939-3130-9_17

Fig. 4.

Fig. 4

Fluorescence microscopy analysis of FGF2 binding to cells remodeled with various neoPGs. Cells treated with AF488-labeled neoPGs display green fluorescence. Cells remodeled with FGF2-binding neoPGs (11D) show significant fluorescence compared to non-binding neoPGs (11A and 11N) upon treatment with exogenous FGF2 and immunostaining with anti-FGF2 and AF555-labeled secondary antibody. Adapted with permission from Huang, M.L., Smith, R.A.A., Trieger, G.W., Godula, K. Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells. Journal of the American Chemical Society (2014), 136, 10565–10568. Copyright (2014) American Chemical Society