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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Methods Mol Biol. 2016;1367:207–224. doi: 10.1007/978-1-4939-3130-9_17

Fig. 5.

Fig. 5

Neural differentiation of neoPG-remodeled Ext1−/− mouse embryonic stem cells remodeled with neoPGs. (a) NeoPG 11D with D2A6 sulfation pattern, with affinity for FGF2, promoted differentiation into neural rosettes, whereas cells treated with neoPGs 11A (b) and 11N (c), and untreated cells (d), retained their embryonic characteristics. (e) Soluble heparin was also found to promote neural differentiation into rosettes. (f) Increasing dosages of neoPG 11D increased neural rosette count. Reprinted with permission from Huang, M.L., Smith, R.A.A., Trieger, G.W., Godula, K. Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells. Journal of the American Chemical Society (2014) 136, 10565–10568. Copyright (2014) American Chemical Society