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. 2019 Jun 17;14(6):e0218509. doi: 10.1371/journal.pone.0218509

Fig 6. CSF seeded RT-QuIC reaction kinetic and PK resistant RT-QuIC reaction products.

Fig 6

(A) Averaged RT-QuIC traces per individual seed are displayed (B) RT-QuIC reaction products from the reaction displayed in (A) were digested with PK 50 ug/mL and probed in WB with mAb SAF70 1:1000. Samples are grouped according to sCJD strain of prions as defined in (8) and sub-grouped according to sCJD subtype as defined in (6). Lane MM: Molecular weight marked; lane rec: 10 ng of FLHa-rPrP; Lane PBS: unseeded reactions; Lane Mix: RT-QuIC untreated reaction mixture.