Fig 2. Aggrecan knockdown ATDC5 chondroprogenitor cells have decreased Alcian blue staining and absorbance levels compared to control cells after ITS stimulation.

Cells were cultured in media containing ITS for 12 days at which they were fixed and stained with Alcian blue. (a) Representative stained cell culture plates are shown for each cell line. (b) Absorbance measurements after Alcian blue extraction are shown as the mean + 1 SD for 3 biological replicates per condition. Hatched bars represent unstimulated cells, and solid bars represent cells stimulated with ITS. *, p<0.01 versus control cells; ** p<0.01 versus control cells +ITS; †, p<0.05 versus control cells.