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. 2019 Jun 17;14(6):e0218399. doi: 10.1371/journal.pone.0218399

Fig 4. Aggrecan knockdown ATDC5 cells are unresponsive to extracellular matrix cues that normally accelerate the chondrogenic program.

Fig 4

qPCR measurements to determine Col2a1 (a) and Col10a1 (b) expression were performed on RNA derived from lentiviral transduced control cells and 2 AggKD cell lines grown in cell culture plates that were untreated (hatched bars) or pre-conditioned with the extracellular matrix of control ATDC5 cells. qPCR measurements to determine Col2a1 (c) and Col10a1 (d) expression were performed on RNA derived from 2 AggKD cell lines grown individually (hatched bars) or co-cultured with wild-type ATDC5 cells. Before RNA extraction, wild-type ATDC5 cells were eliminated through puromycin selection. Data are shown as the mean +1 SD for 4 biological replicates per condition. *, p<0.05 versus corresponding unconditioned samples; **, p<0.01 versus corresponding unconditioned control samples.