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. 2019 Jun 5;17(6):e3000309. doi: 10.1371/journal.pbio.3000309

Fig 4. AMPK modulates the abundance of diverse chromatin marks in the soma and the germ line during the dauer stage.

Fig 4

(A, B) Global levels of H3K4me3, H3K9me3, H3K9ac, and H3K27me3 were quantified by performing whole-animal western blot analysis of daf-2 and daf-2; aak(0) dauer larvae. glp-1(RNAi) was performed postembryonically using dsRNA feeding in order to compromise germline development without affecting early embryogenesis. (C) Global levels of these chromatin marks were quantified using whole-animal western analysis. α-tubulin was used as a loading control to normalize protein levels between samples. Error bars indicate SD from 3 independent experiments. *P < 0.05, **P < 0.001, ***P < 0.0001 using Student’s t test. Underlying data can be found in S1 Data. aak, AMP-activated Protein Kinase subunit; AMPK, AMP Kinase; DAF, DAuer Formation abnormal; dsRNA, double-stranded RNA; glp-1, Germline Proliferation abnormal-1; H3K4me3, histone H3 lysine 4 trimethylation; H3K9ac, Histone H3 Lysine 9 Acetylation; H3K9me3, histone H3 lysine 9 trimethylation; H3K27me3, histone H3 lysine 27 trimethylation RNAi, RNA interference.