Fig. 3.

Effects of various depolarizing agents on [³H]NAPE biosynthesis and [³H]NAE formation in cortical neurons.a, [³H]NAPE biosynthesis: veratridine (20 μm), 4-aminopyridine (4-AP; 3 mm), 3,4-diaminopyridine (3,4-AP; 3 mm), and kainate (0.1 mm). EGTA and tetrodotoxin (TTX) were used at 10 mm and 1 μm, respectively. b, [³H]NAE formation was determined in the same or parallel experiments. The ability of 4-AP, 3,4-AP, and kainate to stimulate [³H]NAE formation was documented previously (Di Marzo et al., 1994). The effect of ionomycin (1 μm, 10 min) is shown for comparison. Results are expressed as percent of control and represent the mean ± SEM of five to eight separate experiments; *p < 0.05, ***p < 0.001 (ANOVA); NS, nonsignificantly different from unstimulated cultures.