Table 2.

NAPE biosynthesis in homogenates of neuronal cultures: lack of incorporation of labeled fatty acids or oleoyl CoA

Conditions	[14C]palmitic acid (dpm ± SEM; n = 3)	[3H]oleic acid (dpm; n = 2)	[14C]oleoyl CoA (dpm; n = 2)
Tissue	864  ± 149	ND	ND
Boiled tissue	790  ± 184	375	90
+ Ca2+	528  ± 57	452	87
+ EGTA	ND	297	38
+ TX-100	477  ± 66	ND	ND
+ Ca2+, TX-100	358  ± 11	ND	ND
+ Ca2+, TX-100, EGTA	543  ± 11	ND	ND

Homogenates were incubated for 1 h at 37°C in 50 mmTris, pH 7.4, containing one or more of the following: Ca²⁺(3 mm), EGTA (10 mm), TX-100 (0.1%, v/v). Results are from one experiment, representative of two experiments performed in triplicate with [¹⁴C]palmitic acid, and from two experiments with [³H]oleic acid and [¹⁴C]oleoyl CoA.