Fig. 1.

Effects of NGF (42 nm) on antimitotic agent-induced human neuroblastoma cell death. A, SH-SY5Y cells were pretreated with NGF for 24 hr, then exposed for 1 hr to 3.2 nm NCS in the presence of NGF.B, NGP cells were pretreated similarly with NGF, then exposed for 1 hr to 2.4 nm NCS in the presence of NGF. In both cases, NGF was maintained in the culture medium for the duration of the experiment. The relationship between cell adherence and apoptosis and the methods for determining adherent cell counts have been described in detail previously (Falcione et al., 1993; Hartsell et al., 1995). In this and the following figures, error bars represent the SEM of three determinations within a single representative experiment and, where not apparent, are smaller than the size of the symbol. All experiments were performed twice, and each repetition gave comparable results to those shown. □, NCS alone; ♦, NCS + NGF; ○, NGF alone; ▵, control.