Fig. 4.

Immunoreactivity for GluR4 at inner hair cell synapses as demonstrated by 15 nm immunogold particles using different fixatives and tissue preparation methods. A, C, D, Fixative No. 2, freeze substitution. B, Fixative No. 4, method ofPhend et al. (1995). Note that some gold particles are associated with the presynaptic membrane. Large arrowhead, Synaptic body surrounded by synaptic vesicles (small arrowheads).D, Enlargement of C. Abbreviations as in Figure1. Scale bars: 0.3 μm in A–C, 0.2 μm inD.