Fig. 4.

a, Uninfected Sf9 cells immunostained for MTs. b, Immunostain for MTs in Sf9 cells 3 d after infection with baculovirus construct encoding N-terminal half of CHO1/MKLP1. c, Immunostain for MTs in an infected Sf9 cell that developed in the presence of 10 nmvinblastine. Arrow shows the cell body, which is nearly devoid of MTs. Inset, The MT array at the midpoint of a process that developed in the presence of vinblastine. Clockwise hooks indicate MTs with their plus ends distal to the cell body, whereas counterclockwise hooks indicate MTs of the opposite orientation. Approximately half of the MTs are of each orientation. Scale bar:a–c, 9 μm; inset, 0.3 μm.