Fig. 3.

NMDA-EPSCs evoked in cerebellar granule cells by stimulating mossy fiber inputs. A, Averaged NMDA-EPSCs recorded from cerebellar granule cells of wild-type (+/+, upper row) and ε1 mutant mice (−/−, lower row) at early (P8, left column) and late (P21–P22,right column) postnatal stages. In Mg²⁺-free solution containing CNQX (20 μm), bicuculline (10 μm), and strychnine (0.5 μm). EPSCs (20–25) were averaged for each trace. Holding potential was −70 mV. B, A kinetic comparison of NMDA-EPSCs. Rise time (10–90%, abscissa) and decay time (from peak to 37%, ordinate) were compared between wild-type (open circles) and ε1 mutant (−/−) mice (filled triangles) at P21–P24.