Fig. 1.
Western blot analysis of huntingtin in adult and developing mouse brain. A, Protein extracts (15 μg/lane) from different areas of the adult mouse brain show that all regions express the huntingtin protein. F ctx, Cortex;WM, subcortical white matter; Cd/put, caudate/putamen; Brnst, brainstem; CBL, cerebellum. B, Protein extracts from whole brain at E10–E17 (Embryonic) and P0–P30 (Postnatal) show the presence of huntingtin at all stages of brain development. The lower molecular weight band that appears in lanes for E15 and E17 may be a degraded product of huntingtin proteolysis. Molecular weight markers are shown on the left.