Fig. 4.

Huntingtin immunoreactivity in the adult mouse brain. a, Sagittal section shows huntingtin labeling throughout the brain and more prominently in the gray matter than the white matter because of the preferential staining of neuronal somata and dendrites. Some folds are present in the cortex of the cerebellum.Arrow identifies the area of cortex shown in band c. Magnification, 4×. b, Huntingtin immunoreactivity is seen in neurons throughout the cortical gray matter. c, Preadsorption control: neuronal staining in cortex is reduced significantly when peptide antigen is added to the anti-huntingtin antisera. Residual huntingtin labeling, which is faintly observed in some perikarya, is probably in neurons that normally express the highest levels of the protein.