Table 2.

Expression of NMDA-R1 subunit in GnRH neurons as detected by immunocytochemistry

	Total # GnRH neurons counted	# NMDAR1-positive GnRH neurons	% NMDAR1-positive GnRH neurons
Prepubertal (P21)
A3	48	1	2
A5	63	2	3
A21	82	0	0
A20	79	1	1
Mean ± SEM	68 ± 8	1 ± 0.4	2 ± 0.6
Pubertal (Day of VO:P36)
A24	52	1	2
A25	68	2	3
A26	46	2	4
A27	73	3	4
Mean ± SEM	60 ± 6	2 ± 0.4	3 ± 0.5
Postpubertal (P41)
A6	60	18	30
A7	50	13	26
A28	67	6	9
A31	61	11	12
Mean ± SEM	60 ± 4	12 ± 2*	19 ± 5*

The number of GnRH neurons that were double-labeled with the NMDA-R1 subunit was determined in prepubertal, pubertal (day of vaginal opening), and postpubertal rats. The identification number of each rat is indicated on the left. Although few GnRH neurons were double-labeled in prepubertal and pubertal rats, this increased significantly in postpubertal rats.

*p < 0.01 versus prepubertal and pubertal.