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Immunoblot analysis of purified MCP and total brain homogenate with anti-MCP polyclonal antibody. Proteins, purified rat brain MCP (MCP), and total brain homogenate (BRAIN) were separated in 14% SDS-polyacrylamide gels and either stained with Coomassie blue R-250 (stained gel) or blotted to nitrocellulose and probed with the anti-MCP polyclonal antibody at 1:200 dilution (Blotα-MCP). A drawing of the complete gel with the migration of appropriate molecular weight standards is shown.
