Activation of an E-Dec neuron by maintained lung inflation and vagus and superior laryngeal nerve stimulation (50 Hz, 3 × threshold) in a rat with intact vagus nerves. A, Lung inflation (increased PTr) prolongs expiratory period and increases E-Dec neuron discharge.B, Vagus nerve stimulation (X stim; 50 μA, 50 Hz) prolongs expiration and increases E-Dec neural discharge similar to lung inflation. C, Superior laryngeal nerve stimulation (SLN stim; 20 μA, 50 Hz) prolongs expiration beyond the period of stimulation and causes a corresponding maintained depolarization of the E-Dec neuron.Arrowheads in B and Cindicate depolarization of the E-Dec neuron in phase with the ventilator-induced lung inflation. All traces are from the same neuron.MP, Membrane potential; ∫Phr, integrated phrenic nerve activity; PTr, tracheal pressure.