Fig. 7.
Low-abundance mRNAs give rise to functional protein. A, Photograph of an ethidium bromide-stained gel in which the amplicons from a single cell have been separated by electrophoresis. Note that this cell expressed detectable levels of D2 and D1b mRNA. B, Whole-cell recording from the same cell showing that the application of the D1 class agonist 6-chloro-PB (5 μm) reversibly reduced Ba2+ currents evoked by a voltage ramp.C, Photograph of an ethidium bromide-stained gel in which the amplicons from another cell have been separated by electrophoresis. Note that this cell expressed detectable levels of D4 but not D2 or D3 mRNA.D, Whole-cell recording from the same cell (fromC) showing that the application of the D2class agonist quinpirole (2 μm) reversibly reduced Ba2+ currents evoked by a voltage ramp.